Methods

Experimental methods

• Biopsies in mammary glands, liver, fat, and endometrium
• B-mode ultrasound
• Milk flow recording
• Intramammary pressure recordings
• Milk cell counting and differentiation

Laboratory methods

• Molecular biology
• qRT-PCR
• Radioimmunoassays and enzymeimmunoassays for various hormones and growth factors
• Primary cell cultures
• Hormone receptor analysis
• Enzymatic determination of metabolites and enzymes